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Thank you for your responses.
It was only a handful of substrate and it was placed directly into my Magnum instead of the carbon. I also placed a small piece of filter material from an established aquarium in it, too. I will remove the substrate from the filter.
I added the substrate to increase the number of beneficial bacteria to the completely new system. My main concern was introducing a lot of new protozoans to the QT tank by adding the substrate to the filter. I assumed that the copper would kill the new protozoans.
The bottom of my aquarium is completely devoid of substrate and only contains some plastic decorations(artificial tree root and some pvc fittings; real realistic) for the fish to hide among. The fish seem calmer since I added these items, and I hope that this will reduce their stress.
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