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Marc, just for giggles do this the next time you culture a batch of phtyo.
Get an recently used and empty 2 gallon container of bleach. Rinse out the container well, a few minutes in warm tap water should be fine. Fill up the container with tap water (unless your water is horrible I get better result with tap than RO/DI). Add a little less than 1/2 cup of salt mix (I try for a SG of around 1.020). Then add 3ml of F/2. Shake the bottle up for about 30 seconds and let is sit at least 24 hours, 48 is better. This is your culture medium.
Use the water from the culture medium the next time you set up some phyto. See if it works. Also set up your bottle with the little piece of cotton as rsman explained. I use one 3/32" hole with a little wedge of cotton in.
When you run the air through the phyto culture, the bubbles should be fast but not fast enough to cause any real bubbling at the top. What I mean by that is, the water at the surface should be turbulent but nothing foamy should be seen.
FWIW, I have better luck starting with live phytoplankton than the disks.
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-Joe
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