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Chinese DNA · #1 07/04/2006, 04:01 AM

I get a trouble in my job. There is a word I don't know in a article I am reading."Preplate the cells for 15min on a collagen coated dish before moving the cells remaining in suspension to a new collagen coated dish". What is"Preplate" meaning? Thank you very much.

ShellBell · #2 07/04/2006, 04:08 AM

Maybe you mean palpate - which means to examine by touch especially medically

Just a thought!

Chinese DNA · #3 07/04/2006, 04:11 AM

I am reading "Isolation and growth of mouse primary myoblasts"

Habib · #4 07/04/2006, 04:20 AM

Preplate technique allows the seperation of cells based on their differences in adhesion characteristics.

Preplate is a technique and the media used for adherence might vary with which cells one would like to seperate.

ShellBell · #5 07/04/2006, 04:24 AM

Quote:

Originally posted by Habib
Preplate technique allows the seperation of cells based on their differences in adhesion characteristics.

Preplate is a technique and the media used for adherence might vary with which cells one would like to seperate.

Sounds good to me!!

Chinese DNA · #6 07/04/2006, 04:27 AM

To Habib: I am sorry for my poor English. what is "seperation" and "seperate" meaning? thank you very much

Habib · #7 07/04/2006, 04:36 AM

Quote:

Originally posted by Chinese DNA
To Habib: I am sorry for my poor English. what is "seperation" and "seperate" meaning? thank you very much

I didn't spell correctly.

separation and separate.

http://dictionary.reference.com/browse/separation

Habib · #8 07/04/2006, 04:37 AM

Quote:

Originally posted by ShellBell
Sounds good to me!!

Otherwise I would have changed your name back to what it was.

ShellBell · #9 07/04/2006, 05:52 AM

Quote:

Originally posted by Habib
Otherwise I would have changed your name back to what it was.

- I tried!

Sk8r · #10 07/04/2006, 10:18 AM

Perhaps looking at it as pre [before] + plate?

newty · #11 07/04/2006, 10:31 AM

It means this:

1. Add x concentration of y volume collagen to plate for 15 min. Wash it off with preferred solution (DPBS or media without additives - it eliminates the acidicity of collagen) or dry completely.
2. Remove an aliquot of the cells to said plate. Ususally it is trypsinized first. Primary myoblasts grow as adherant cells, not cells in suspension.
3. Incubate with myoblast media. It can contain serum or not. Check the paper to make sure. I have used both situations. It depends on your media components.

Cheers, Shawn

newty · #12 07/04/2006, 10:34 AM

Quote:

Originally posted by Habib
Preplate technique allows the seperation of cells based on their differences in adhesion characteristics.

Preplate is a technique and the media used for adherence might vary with which cells one would like to seperate.

That is true, but I believe that in this case all he needs to do is coat the plate with collagen to make sure that myoblasts stick to the tissue culture plate.

Preplating with certain substances can preferentially remove or retain cells of interest. I use this technique to a certain degree to isolate retinal ganglion cells (from the eye) to get rid of contaminating macrophages. The macrophages stick to any surface within 20 minutes while my RGCs stay in suspension during this time. It allows me to further purify my RGCs.

Shawn

Habib · #13 07/04/2006, 01:51 PM

That is true,

Not bad for a chemist, he?