Nannochloris starter culture?

mwp · #1 11/09/2006, 12:27 PM

NOT Nannochloropsis, and not from FAF (who says they are completely out of Nannochloris disks AND live cultures). Kathy's Nannochloris got contaiminated, and I don't know if I passed it along to anyone else.

So...anyone know where I can get a new starter culture for my Nannochloris? Dan at SHS is looking, but I'm getting to that point where I need to start widening my net.

I should've NEVER dropped them from my culture group!

Matt

SueQ · #2 11/09/2006, 02:02 PM

Matt-
Here are 2 places:
http://www.zo.utexas.edu/research/utex/

http://ccmp.bigelow.org/

Neither are cheap

And they'll both ship liquid cultures. The stuff we've ordered from TX has arrived a bit more dilute than we'd like to see, but they're "cheaper". CCMP will cut you a deal if you order through an academic institution, so if you can get someone in a local university to order if for you you'll only pay ~$45 plus shipping, I think...
I have finally found a cheap source for Rhodomonas, Nannochloropsis, Tetraselmis and Iso. But, not any other cool phytoplankters..

mwp · #3 11/09/2006, 03:15 PM

Yeah...had both of those up in the web browser last night...awefully expensive...maybe if I still haven't found them cheaper and I've sold a bunch of percs I can bite the bullet

Matt

Kathy55g · #4 11/09/2006, 03:56 PM

just curious, why do you want that particular one?

Kmiec123 · #5 11/09/2006, 04:50 PM

Matt, Not sure what you mean( I can't read all that well, But if you do want a micro algea disc from faf I have an extra/backup on and all cuture going good right know I will be willing to part with. PM mew if interested. Carl

mwp · #6 11/09/2006, 05:05 PM

Kathy - Mandarins..that's all I can say

If you have an extra NANNOCHLORIS disk Carl, I would be MORE than interested in it. Or, for that matter, if you wouldn't mind helping me out with maybe 100L of live, ready to go, by all means that's what I'm looking for!

PM sent!

Matt

Matt

mwp · #7 11/09/2006, 06:37 PM

Ah nuts. Carl, thanks for the call. Unfortunately, what carl had turned out to be Nannochloropsis. SOOO...I'm still on the hunt for Nannochloris...anyone have it around in culture right now?

Matt

mwp · #8 11/13/2006, 08:25 PM

STILL looking for a Nannochloris starter if anyone has it. Dan @ Seahorsesource didn't have any luck. I'm trying a few other avenues, but at the moment, I'm coming up phyto-less

Matt

mano · #9 11/14/2006, 12:05 AM

Maybe try a shellfish lab to see if they could spare some? I can't think of a particular bivalve right now that might eat it but maybe search a couple and see if any farms are using it. I don't know where else to look other than a university with an aquaculture program maybe?

Christine

mwp · #10 11/14/2006, 12:10 AM

Interesting Idea. I do know of at least 2 Phytoplankton "warehouses" that are maintained by academia...the issue is that as a private individual, a 25 ML innoculant runs about $75 plus shipping

Matt

mano · #11 11/14/2006, 12:20 AM

Someone on ebay claims to have some...

http://cgi.ebay.com/ws/eBayISAPI.dll..._promot_widget

mwp · #12 11/14/2006, 01:04 AM

MANO - you rock! Thanks! Emailing the guy/gal now!

Matt

mano · #13 11/14/2006, 08:55 PM

I hope it works out Matt!

Christine

David M · #14 11/15/2006, 12:18 AM

Gotta wonder about a guy who balks at a $75 culture he thinks will grow out 100's of $90 fish

mwp · #15 11/15/2006, 01:25 AM

OH David, those are FIGHTIN' Words, the type that get me to figure out how to do the mandarins WITHOUT Nannochloris (it's looking that way so far).

Long story short, I'm still very low on the funds at the moment post-deer accident, and lately I have to justify a fair amount of purchases (and rightfully so considering the near future financial situation we'll be in). Hard to buy at $75 culture when you techinically don't have the cash on hand..

Matt

aomont · #16 11/15/2006, 01:18 PM

Maybe we could help you find a substitute if we knew why it´s so important !

Know it´s secret but couldn´t help it .

Any answer from the guy/girl on e-bay ?
Anderson.

mwp · #17 11/15/2006, 02:24 PM

Nope, no word from the eBayer

Matt

tmays · #18 11/15/2006, 02:25 PM

You might want to try AlgaGen.com they have alot of live cultures of phyto and zooplankton sp. Make sure it is for personal use and not resale.

mwp · #19 11/15/2006, 02:37 PM

I did contact them...waiting to hear back.

Matt

tmays · #20 11/15/2006, 02:50 PM

I hope you get a hold of what your looking for, and good luck with it!

mwp · #21 11/15/2006, 10:55 PM

Well, eBay guy (David) cultures Tetralsemis, Nannochloropsis and Nannochloris all together, so there's no way to start a pure Nannochloris culture with what he currently has.

Still on the hunt...

Matt

aomont · #22 11/15/2006, 11:07 PM

Did he said that ? Thougth he cultured them separetly and put together to sell...
Anderson.

mwp · #23 11/16/2006, 02:01 AM

Hope not to get anyone in trouble or anything, but straight from the email...

Quote:

At the current, I dont have a seperate culture, but will be ordering new cultures this weekend....I used to keep them seperate, but I have found they all grow well together, so I have been doing it that way. Much simpler than keeping seperate.

So the jury is out...maybe he has a line on Nannochloris, and maybe we can do a trade for some T-Iso.

I also received another avenue to try this evening...will try it in the morning!

Matt

Kathy55g · #24 11/16/2006, 08:16 AM

trouble with culturing together, is they all compete for the same resources, and the strongest culture will win. When you got 3 green phytos, how will you know what you have?

Kathy55g · #25 11/16/2006, 08:19 AM

In fact, this summer I was growing some of Matt's Isochrysis, and it turned from golden to green, so I knew the jig was up. I had an algal contamination that I would not have noticed in a green culture, which is everything else I am culturing (green colored algae).

I am thinking of carrying an iso culture at all times, to be the canary in the coal mine, and tell me when I need to start over with fresh cultures.