it's not about living things in the beach sand, it's about polution. stuff in the sand will decompose or leech, causing your levels to rise. the point of a DSB is to reduce levels. it's not good to add more into the system taht it will have to overcome
sand is cheap. You don't have to have any special kind. $5/bag play sand at HD will do just fine. you'd probably only need one bag...two at most for a 5G bucket
as for rinse and reuse, you have to worry about stirring up the bad stuff that stays at the bottom of a sand bed. here again, sand is cheap so you could just start over. Might even want to run the two simultaneously for a month or two so that the new bucket can get seeded and you aren't just removing all the anerobic bacteria at one time.
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