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Most of the posts that I read said 1 month for the bacteria to colonize and the results to start showing up.
This probably depends, however, on the amount of nitrates you have.
My nitrates we around 10-20 also on my 40 gallon tank. I just hooked it up a week ago, so we'll see when I start to see result, although I have been doing more-than-normal water changes, so it would be hard to tell if the bucket is helping at all.
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