I believe I was using up to 3.5 ml vodka in a 29g softie tank for 6 months or longer with no problem. The key is simply going slow, and not increasing vodka more than .25 ml every 2-3 days. Never had a bloom with potential 02 deprivation doing it that way, and softies grew just great and even the poorly performing AquaC HOB skimmer pulled out an impressive amount of gunk.
I did eventually dial back to about 1.5 ml as lowest effective dose.
I think a 400g system could take a lot more than 4 ml daily. I don't think you'd even get close to a bloom with that as initial dose.
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