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-   -   Microfood culture: phytoplanktons, Rotifers, ciliates, Artemia, and copepods (https://archive.reefcentral.com/forums/showthread.php?t=135137)

GreshamH 04/20/2006 11:59 AM

It's an "L" type. "s" and "ss" are [i]B. rotundiformis[/i].

rutledgek 04/20/2006 12:35 PM

So I would want a different type for raising them to feed to Clown larvae.

NicoleC 04/20/2006 01:14 PM

B. plicatilus is perfect for clownfish.

rutledgek 04/20/2006 01:22 PM

Thanks for you help. What species of fish would the s and ss type be used for.

NicoleC 04/20/2006 01:29 PM

Anything with very small fry, like small gobies. The B. rotundiformis is a bit smaller than B. plicatilus.

rutledgek 04/20/2006 01:32 PM

Thanks for the help. I just wanted to make sure before I bought the wrong live food.

rutledgek 04/22/2006 04:46 PM

One last question. Does anyone know the size of rotifer cysts are?

rutledgek 05/24/2006 03:23 PM

Is there anyone with a calanoid culture that they can send me. I am looking to start a culture of these for dwarf seahorses.

spawner 05/24/2006 06:01 PM

Email Erik at Algagen: [email]eriks@algagen.com[/email]

Do you have a good cutlure of microalgae? You'll need at least Iso; but would do better with a diatom as well. Nanno won't work.

rutledgek 05/25/2006 12:03 PM

[QUOTE][i]<a href=showthread.php?s=&postid=7431589#post7431589 target=_blank>Originally posted</a> by spawner [/i]
[B]Email Erik at Algagen: [email]eriks@algagen.com[/email]

Do you have a good cutlure of microalgae? You'll need at least Iso; but would do better with a diatom as well. Nanno won't work. [/B][/QUOTE]

Nanno is suggested in the copepod culturing article at the beginning of this thread.

spawner 05/25/2006 01:57 PM

Nanno will not work for calanoid copepods, the seems to be a lot of misunderstanding about the different types of copepods and their food requirements.

Teremei 06/23/2006 12:15 PM

I just got myself a nice fancy stand mounted hatchery with drain on the bottom. I was so excited, I started my first culture. They are just starting to hatch now. If I read that guide right, I shouldn't even worry about feeding them for another 8 hours, because their mouth and anus aren't fully developed. Is this correct?

This is baby brine by the way. .

spk 06/25/2006 04:49 AM

Yes that is correct. The mouth and anus develop later. If you a feeding to fry, feed just after hatch.

Steve

mwp 07/14/2006 02:25 AM

There's probably stuff like this already in the thread, but hey, maybe not? Below are some pix and vids I shot with the QX5 for the Mandarin Thread ([url]http://archive.reefcentral.com/forums/showthread.php?s=&postid=7740361#post7740361[/url]) - in a nutshell, I sampled 1 L-Strain Rotifer Culture and 1 SS-Strain Rotifer Culture. Seived both through 53 micron and seived the flow-through in a secondary 10 micron seive. It's really worth noting the amount of TINY life prospering in the L-Strain Culture; meanwhile the SS-Strain culture had virtually NOTHING other than rotifers....not even worth showing the pics and vids of what was collected via the 10 micron from the SS-Sample...again, literally NOTHING.

One other note, I chilled the samplings in the freezer for a minute or two to SLOW these tiny creatures WAY DOWN...normally they're almost a blur under the microscope, especially at 200X.

Videos are .avi; roughly 1 to 2.5 mb a shot.

[b]L-Strain Rotifers at 60X - numerical measurments are 1 MM[/b]
[img]http://www.cichlidrecipe.com/nanoreef/L_Strain_Rotifers_60Xb.jpg[/img]
Video - [url]http://www.cichlidrecipe.com/nanoreef/L_Strain_Rotifers_60X_chilled.avi[/url]

[b]SS-Strain Rotifers at 60X - numerical measurments are 1 MM[/b]
[img]http://www.cichlidrecipe.com/nanoreef/SS_Strain_Rotifers_60Xb.jpg[/img]
Video - [url]http://www.cichlidrecipe.com/nanoreef/SS_Strain_Rotifers_60X_chilled.avi[/url]

[b]L-Strain Rotifers at 200X - numerical measurements are 200 um (microns, micrometers etc..)[/b]
[img]http://www.cichlidrecipe.com/nanoreef/L_Strain_Rotifers_200Xb.jpg[/img]
Video -
[url]http://www.cichlidrecipe.com/nanoreef/L_Strain_Rotifers_200X_chilled.avi[/url]

[b]SS-Strain Rotifers at 200X - numerical measurements are 200 um (microns, micrometers etc..)[/b]
[img]http://www.cichlidrecipe.com/nanoreef/SS_Strain_Rotifers_200Xb.jpg[/img]
Video - [url]http://www.cichlidrecipe.com/nanoreef/SS_Strain_Rotifers_200X_chilled.avi[/url]

[b]L-Strain Culture, smaller than 53 microns but larger than 10 @ 60X:[/b]
[img]http://www.cichlidrecipe.com/nanoreef/L_Strain_less53micron_60Xb.jpg[/img]
Video - [url]http://www.cichlidrecipe.com/nanoreef/L_Strain_less53micron_60X_chilled.avi[/url]

FWIW,

Matt

jake levi 07/20/2006 09:07 AM

Frank do you know what they were culturing and feeding the Flame Angel larvae in Hawai ?

I am told they were half the size of rotifers. Personal communications with Martin Moe said his efforts were stalled out not having anything smaller then rotifers the first several days. He even spent time trolling with a plankton net trying to get enough small plankton to feed. But they starved out.

Any suggestions ?

BTW, a simple culture system I ran across at Scripps used f gal water jugs with bottoms removed, taps siliconed in the necks with airstones, and mounted in rack to make arrays, great for phytoplnakton, rotifers and artemia. You can grow a lot in this system. MUST keep rotifers from contaminating the phyto !

jake

GreshamH 07/20/2006 11:46 AM

If you do a search on RC, that information has been posted about the flames.

Luis A M 07/20/2006 12:45 PM

Hi Jake,you´re the same ol´Jake of BR list?.:)
Yes,the species of pods Baensch (and other Hawaiians) used were posted,published lots of times.One of his articles in FAMA gives names and pics.
I always felt Martin deserved to be the first to crack pigmies.He worked a lot with cherubs in BR days and he was using pods.And he knows how to use them,how you think he raised large angels in the 70´s?.

jake levi 07/20/2006 03:11 PM

laval food for centropyge
 
Hi Luis
yup, same ol same ol, but been out of the country awhile,

just getting re-setup,

I am sure Martin would have been first but it conflicted with getting his book finished and to the printer so he couldnt put the time into them that he wanted to,

do you know which back issue of FAMA ? I am ordering some anyhow. I'll see what I can find here.

how are your projects doing ?

jake

Luis A M 07/21/2006 01:17 AM

Re: laval food for centropyge
 
[QUOTE][i]<a href=showthread.php?s=&postid=7780588#post7780588 target=_blank>Originally posted</a> by jake levi [/i]
[B]Hi Luis
yup, same ol same ol, but been out of the country awhile,

just getting re-setup,

I am sure Martin would have been first but it conflicted with getting his book finished and to the printer so he couldnt put the time into them that he wanted to,

do you know which back issue of FAMA ? I am ordering some anyhow. I'll see what I can find here.

how are your projects doing ?

jake [/B][/QUOTE]

Good to have you back,Jake,you enjoyed diving the Red Sea?
Doing fine,thanks, always working new projects:)
FAMA 25(12):4-12 and especially 26(7):156-162

polypboy 08/03/2006 09:02 AM

Haven't ever done it so can't really comment but looks like copepod culture wouldn't be too much of a nightmare. How would one go about separating nauplii from other stages? Would you just have to maintain a very large culture and then strain through suitably sized media to get suffient numbers of sufficiently small nauplii?

Luis A M 08/03/2006 09:49 AM

That´s right.You´ll need lots of phyto,though.

polypboy 08/03/2006 11:00 AM

Thanks. How big a culture do you think you'd need to provide a single hatch of centropye larvae with copepod nauplii through to ful size pods or rotifers? I guess this would depend largely on the culture density but are we talking 10 gallon tank or bathtub?

GreshamH 08/03/2006 11:21 AM

Depends on what copepods we're talking about. The ones I have the most experience with (Tigriopus californicus) would require quite a few 10g tanks, some 55ul, 100ul and 250-400ul screens. Do read up on the nutritional requirements of the particular copepod species your dealing with though, somes growth is inhibited by certain phytoplanktons.

spawner 08/03/2006 01:25 PM

Those aren't real copepods Gresham, You can't many culture any fish on harpacs. They are not worth much in regards to larval feeds, some will even eat your larvae.

Calanoids are very different, require much stricter culture protocols and food. You'd need a 20L or so of top quaility algae a day to maintain a good calanoid culture; and that is at small scales.

GreshamH 08/06/2006 12:16 PM

I can't many culture? At small scales? Tired and overworked? :D

So your saying herpacs are not real copepods? Do they know this? :lol:

Understood that not many will eat the hairy herpacs, but the system I use to grow them, was bastardized from a calanoid set-up, with a few modifications (screen sizes/vertical walls of window screen/feed) ;)

Do you not distpute that certain algaes have shown to inhibit growth, even act as a prophylactic?


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